Illumina Sample Prep

RNA-seq

The GC3F is now offering Illumina library preparation services for RNA-sequencing libraries using the Kapa Biosystems Stranded mRNA-seq Kit.

Our RNA-seq sample prep service includes:
*QC and quantification of starting total RNA by AATI Fragment Analyzer
*Barcoding of multiple libraries for multiplexing
*qPCR quantification of each barcoded sub-library
*Final pooling based on qPCR quantification results
 
Requirements:
0.1 to 4 µg of high quality total RNA (RIN > 8*) in < 50 µL of RNase-free water or buffer
 
RNA-seq Sample Prep Pricing:
Internal Rate (UO/OSU/OHSU):  $155 per sample
External Rate:                                 $255 per sample

Contact Maggie Weitzman (mweitzm2@uoregon.edu) for details on sample drop off, etc.

*Libraries can be prepared from degraded RNA. We will let you know if your RNA looks degraded and discuss options for moving forward.

Whole Genome DNA

We can prepare whole genome Illumina shotgun libraries from your DNA samples using the Kapa Biosystems HyperPlus kit.
 
Both conventional and PCR-free workflows are available
 
Requirements:
Conventional amplified prep: 50 ng to 1 µg of pure, high molecular weight gDNA in < 35 µL of 10 mM Tris pH 8.5
PCR-free prep: 0.5 µg to 1 µg of pure, high molecular weight gDNA in < 35 µL of 10 mM Tris pH 8.5
 
*Both protocols are inhibited by the EDTA found in some popular DNA storage buffers (like TE).  DNA should be submitted in 10 mM Tris pH 8.5 (Qiagen EB buffer).
 
Genomic DNA Sample Prep Pricing:
Conventional Internal Rate (UO/OSU/OHSU):  $140 per sample
Conventional External Rate:  $230 per sample
 
PCR-free Internal Rate (UO/OSU/OHSU):  $165 per sample
PCR-free External Rate:  $255 per sample
 
Contact Maggie Weitzman (mweitzm2@uoregon.edu) for details on sample drop off, etc.