The GC3F houses numerous instruments for DNA, RNA, and protein quantification.
All the quantification instruments listed on this page are free to use by the UO community - just bring your own reagents and consumables!
- The ABI StepOne Plus, BioRad CFX96, and Microplate Reader require sign-up to reserve your time slot in advance.
- The Nanodrops, Qubit, and Licor do not require reservations.
ABI StepOne Plus
The GC3F manages an Applied Biosytems StepOne Plus real-time qPCR instrument.
Utilizing robust LED based 4-color optical recording, the StepOne Plus is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
Please note that due to high popularity, there is a time limit of max 4 hours per researcher per day on the ABI StepOne Plus instrument.
NOTE TO USERS: If upon attempting to launch a new experiment you get a warning that there are uncollected results on the instrument, they belong to the previous user and will be overwritten unless you save them first!
Please do one of the following before proceeding with your run:
- Save the previous results to a flash drive, OR
- Download the previous results to a networked computer
- Then, contact the GC3F and/or the previous user to let us know.
NOTE TO USERS: The new IP address for the Applied Biosystems StepOne Plus instrument is 18.104.22.168 (as of 12/06/17)
The GC3F manages a BioRad CFX96 real-time PCR instrument.
This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.
This instrument also comes with High Resolution Melt (HRM) curve analysis software!
NOTE TO USERS: This instrument is designated for use by members of META from 8am-12pm, Monday through Friday. It is open for booking by any user after 12pm during the week, and any time outside business hours.
If you’re not from META and would like to use this instrument in the morning during the week, you are welcome to book a morning time slot as long as no one from META has already booked it by that morning.
Licor Odyssey Fc Imaging System
The Licor Odyssey Fc analyzes both chemiluminescence and near-infrared fluorescence such as Western blots, agarose gels, SDS-PAGE gels, Coomassie protein gels, RNAi analysis, and other assays. It uses 2-channel near-infrared fluorescence (green & red signals), which allows 2 proteins to be detected in the same sample lane without stripping and re-probing. Direct detection of near-infrared fluorescence signals is performed using secondary antibodies labeled with IRDye NIR fluorescent dyes, or other commercially available dyes such as SYBR Safe, SYBR Green I, SYTO 60, and other popular DNA stains.
The Licor exhibits 6 logs of linear dynamic range, meaning both strong and faint bands can be accurately documented in just 1 scan without image saturation. Digital imaging eliminates the need for processor chemicals, developer maintenance, and x-ray film!
Molecular Devices SpectraMax M5E Microplate Reader
The M5e Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence intensity measurements, glow luminescence, time-resolved fluorescence, fluorescence polarization, and HTRF. It accepts cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Other standard features include spectral scanning across the wavelength range in 1 nm increments, up to six absorbance wavelengths per read, and up to four wavelength pairs for other read modes.
Qubit 3.0 Fluorometer
The Qubit 3.0 Fluorometer is a popular benchtop fluorometer (with a fancy touch screen!) that measures the concentration of DNA, RNA, and proteins via reporting by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result.
The GC3F houses two Nanodrop instruments (the 1000 and the 2000) for quantification of proteins and nucleic acids. Thermo Scientific's patented sample retention system allows for the analysis of 1-2 µl samples, without the need for cuvettes, capillaries, or loading dyes.