The GC3F began offering Illumina sequencing services in the early days of next-generation DNA sequencing in 2008. Since then we have sequenced literally thousands of samples on multiple Illumina platforms. We played a significant role in the development of the RAD-seq technique that was invented at UO, and are still happy to sequence “unconventional” libraries on our instruments.
QC for all single tubes submitted for Illumina sequencing is included in the sequencing request. Sequencing requests which require QC of multiple individual tubes for multiplexing incur a small additional charge per tube.
NovaSeq 6000
GC3F’s workhorse Illumina sequencer, purchased thanks to a generous award from the Murdock Charitable Trust. NovaSeq flowcells consist of multiple lanes, all of which must be full for a run to launch.
NovaSeq 6000 offers 3 run sizes which can be run in a variety of configurations:
- S4 lane: 2-2.5 billion read pairs
- S1 lane: 650-800 million read pairs
- SP lane: 325-400 million read pairs
These are the official Illumina output specs and are conservative. Real world performance will often exceed these read numbers.
NovaSeq Submission Requirements
Please submit 25 µL minimum volume of your libraries at 2-20 nM concentration in a standard DNA storage buffer, such as Qiagen EB.
NextSeq 2000
The NextSeq 2000 is the latest medium output sequencer from Illumina. One user-submitted library is loaded per run, so there is never any waiting for additional libraries to arrive in the facility to fill flowcells.
NextSeq currently offers 3 output modes:
- P3 flowcells yield ~1.2 billion read pairs
- P2 flowcells yield ~400 million read pairs
- P1 flowcells yield ~100 million read pairs
NextSeq Submission Requirements
Please submit 25 µL minimum volume of your libraries at 2-20 nM concentration in a standard DNA storage buffer, such as Qiagen EB.
MiSeq
The Illumina MiSeq is a benchtop instrument designed for lower-output sequencing applications such as amplicons (targeted resequencing), small genomes, miRNA & small RNA, and targeted gene expression profiling.
In addition to offering both single read (SR) and paired end (PE) read types, it can be operated in 4 different output modes to help you narrow in on the ideal configuration for your experiment:
- v3 mode: Up to 25M reads at max read length of 300bp
- v2 mode: Up to 15M reads at max read length of 250bp
- Micro mode: Up to 4M reads at max read length of 150bp
- Nano mode: Up to 1M reads at max read length of 250bp
MiSeq Submission Requirements
Please submit 25 µL minimum volume of your libraries at 2-20 nM concentration in a standard DNA storage buffer, such as Qiagen EB.