GC3F-EA-151 - Abernethy_Lane1 - 2b-RAD

Run Info

Start / End: -

Submitter Info

Lab: Lytle, David (OSU) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
Length Preferred
75

Details

Fragment Size From
170
Fragment Size To
170
Volume Submitted (µL)
25
Measured Concentration
7.00
Method for Measuring Concentration
Fluorometer
Primer Kit
Custom
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
I got 7 ng/ul when I quantified the pooled library using the Flourometer, which should be 170bp total (with 2b-RAD, I have 36 bp that I want sequenced, plus all the identifying fragments attached to the ends).

Library concentration = 7 ng/µL
Median size fragment = 170bp
Library molecular weight = 170bp × 650g/mol per bp = 110500g/mol
Molar concentration = 7 ng/µL / 110500ng/nmol = 0.0000633nmol/µL
= 0.0633µM
= 63.3 nM

This would be more than the 20nM requested. Can you dilute after your QC?

As for the primers/barcodes/indexes, I think I filled out the info correctly. I'm not sure about the kit used. I have attached the sequences for the unique HT/BC codes that I attached to each individual library.

iLab

iLab Service ID
GC3F-EA-151 - Abernethy_Lane1 - 2b-RAD
iLab State
completed
Date Request Submitted
Lab
Lytle, David (OSU) Lab