GC3F-AK-361 - Klocko_UCCS_08/2018 - Hi-C

Run Info

Start / End: -

Submitter Info

Lab: Klocko, Andrew (UCCS) Lab

Service

Service Requested
HiSeq 4000
Read Type
Paired-End
Length Allowed
75
100
Length Preferred
100

Details

Fragment Size From
200
Fragment Size To
1000
Volume Submitted (µL)
25
Measured Concentration
1.30
Method for Measuring Concentration
Fluorometer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
Hi Doug, This is not my best library prep, but we specifically wished to resequence some of our past samples that were amplified with less PCR cycles during the barcoding process. As such, I didn't have as much material to use for my dilutions. My math says I am at a concentration of 3.33nM (again, lower than I like to give you) but I want to focus on three of these samples, which had less material (around 270M for these three). I am providing the 25uL minimum, but I do have an additional 25uL here in case something goes horribly wrong.

Also, please note that I did not get a chance to run a gel to completely confirm the sizes of the library given the low material yield; if during the quality control stage you find that the range of library fragments is not between 200-1000, please contact me immediately and we can discuss whether to proceed. I believe we should have removed any fragments lower than 200bp, given that we used AmpureXP beads to purify the DNA from our PCR reactions prior to pooling. I noted above that the size range was between 200-1000bp because this was the size range of the previous library that we barcoded (and sequenced in early August), and since we used the same material and the same protocol (minus seven PCR cycles) I am assuming the library will have the same size range. But please double check when you do the QC of the library (I assume you will do a size range analysis).

iLab

iLab State
completed
Lab
Klocko, Andrew (UCCS) Lab