Service
Service Requested
HiSeq 4000
Read Type
Paired-End
Length Allowed
75
100
Length Preferred
100
Details
Fragment Size From
200
Fragment Size To
1000
Volume Submitted (µL)
25
Measured Concentration
1.30
Method for Measuring Concentration
Fluorometer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Klocko, Andrew (UCCS) Lab
Also, please note that I did not get a chance to run a gel to completely confirm the sizes of the library given the low material yield; if during the quality control stage you find that the range of library fragments is not between 200-1000, please contact me immediately and we can discuss whether to proceed. I believe we should have removed any fragments lower than 200bp, given that we used AmpureXP beads to purify the DNA from our PCR reactions prior to pooling. I noted above that the size range was between 200-1000bp because this was the size range of the previous library that we barcoded (and sequenced in early August), and since we used the same material and the same protocol (minus seven PCR cycles) I am assuming the library will have the same size range. But please double check when you do the QC of the library (I assume you will do a size range analysis).