Service
Service Requested
HiSeq 4000
Read Type
Paired-End
Length Allowed
100
Length Preferred
100
Details
Volume Submitted (µL)
~30-35
Measured Concentration
2.00
Method for Measuring Concentration
Fragment Analyzer
Primer Kit
Custom
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Winuthayanon, Wipawee (WSU) Lab
1) I Ctr, I 0.5d, I 1.5d, I 2.5d for one sequencing lane. (these are from mouse's isthmus epithelial cells)
2) A Ctr, A 0.5d, A 1.5d, A 2.5d for another sequencing lane. (these are from mouse's ampulla epithelial cells)
Each sample was loaded with targeted ~8,000 of cells. I would like to recover an approximately equal amount of reads from all samples. Please see the Fragment analysis files attached - all the concentrations in FA report are at 1:10 dilution. Can you please pool them for me? The additional cost for pooling is okay with me.
I will be shipping the sample tomorrow. If you have any questions, please email "Joy" directly at w.winuthayanon@wsu.edu Thank you!