GC3F-GM-551 - AM - RRBS

Run Info

Start / End: -

Submitter Info

Lab: Martovetsky, Gleb (AM) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
100
Length Preferred
75

Details

Fragment Size From
200
Fragment Size To
500
Volume Submitted (µL)
25
Measured Concentration
10.00
Method for Measuring Concentration
Fluorometer
Primer Kit
Illumina TruSeq kit for DNA (24 indices)
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
Yes

Sample

Expected Date Sample will be Submitted

Payment

Comments
Whether you spike in read 1 primer or replace it, up to you. Whatever you did for GC3F-GM-347 worked well. I don't know what % PhiX you used (to help with base complexity since these are RRBS samples), but just do whatever you did last time. Thanks!

I saw your sequencing queue, and it looks like you need 1 lane for SE100 and 3 lanes for SE75 to complete flowcells. Ideally you would run SE75 for 3 lanes, 1 lane at SE100 and trim to SE75, and charge for 4 lanes of SE75. If necessary, I suppose we could pay for one lane of SE100, even though we would only use SE75 to keep read length consistent.

iLab

iLab Service ID
GC3F-GM-551 - AM - RRBS
iLab State
completed
Date Request Submitted
Lab
Martovetsky, Gleb (AM) Lab