GC3F-AS-685 - Scml2KO_IR_F1_sperm_RNA-seq - mRNA-seq

Run Info

Start / End: -

Submitter Info

Lab: Namekawa, Satoshi (CCHMC) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
Length Preferred
75

Details

Volume Submitted (µL)
25
Measured Concentration
5.00
Method for Measuring Concentration
Fluorometer
Primer Kit
Illumina Nextera kit for DNA (96 indices)
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
I attached library sample information (8 samples), I will send samples with dry-ice. Firstly, could you please perform "QC/multiplexing of completed libraries + size selection/concentration prior to sequencing to extract 200-1000 bp range of each library. After excision of 200-1000 bp range and calculating molar concentration of respective libraries, please pool all library samples to 1 tube at equal molar concentration (ex; 10 nM each), to get the equivalent numbers of sequence reads from each library sample. I will submit this Sample prep. request form also.

iLab

iLab State
completed
Lab
Namekawa, Satoshi (CCHMC) Lab