This library contains 30 samples that were dual indexed using 30 unique i5 and 30 unique i7 indexes for a total of 900 possible index combinations (to examine tag-switching). If possible, please use all 900 index combinations to demultiplex reads on the HiSeq 4000. The .csv file containing all possible index combinations was emailed to Doug on 11/30/2018.

I measured the library concentration by Qubiting to determine ng/ul concentration, running on TapeStation to determine mean fragment size, then using this equation (https://support.illumina.com/bulletins/2016/11/converting-ngl-to-nm-when-calculating-dsdna-library-concentration-.html) to calculate nM concentration. The library was then diluted to 10 nM concentration using Illumina's pooling calculator (https://support.illumina.com/help/pooling-calculator/pooling-calculator.htm). I ran the final library on an HS D1000 TapeStation ScreenTape - I have attached the report from that run to this form.

Thank you and happy holidays!