Service
Service Requested
HiSeq 4000
Read Type
Paired-End
Length Allowed
150
Length Preferred
150
Details
Fragment Size From
200
Fragment Size To
1200
Volume Submitted (µL)
45
Measured Concentration
2.00
Method for Measuring Concentration
qPCR
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
IBEST Genomics Resources Core
I will make a request on my end for a PO for all 7x lanes of 2x150 HiSeq 4000, 1x qPCR, and 1x FA.
The 14x HiC libraries nearly all were 1,000bp after a dual-sided size-selection.
Warning: The distribution of the pooled libraries may be slightly bimodal; 6x PCR-free libraries may have shorter average insert-length than average 14x HiC libraries despite the HiC libraries receiving a dual-sided bead-based size selection (0.8x/0.55x).