GC3F-DN-795 - TS 20lib pool - HiC, Shotgun, and PCR-free pool

Run Info

Start / End: -

Submitter Info

Lab: IBEST Genomics Resources Core

Service

Service Requested
HiSeq 4000
Read Type
Paired-End
Length Allowed
150
Length Preferred
150

Details

Fragment Size From
200
Fragment Size To
1200
Volume Submitted (µL)
45
Measured Concentration
2.00
Method for Measuring Concentration
qPCR
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
I will attach a sample-submission sheet hopefully today for all 20 libraries which exist in a single tube and are supped to be split across seven lanes.

I will make a request on my end for a PO for all 7x lanes of 2x150 HiSeq 4000, 1x qPCR, and 1x FA.

The 14x HiC libraries nearly all were 1,000bp after a dual-sided size-selection.

Warning: The distribution of the pooled libraries may be slightly bimodal; 6x PCR-free libraries may have shorter average insert-length than average 14x HiC libraries despite the HiC libraries receiving a dual-sided bead-based size selection (0.8x/0.55x).

iLab

iLab State
completed
Lab
IBEST Genomics Resources Core