I am not sure how the dual-indexed HyperPrep ligated adpators are sequenced on HiSeq4000...I will look into this...though ideally someone would say "hey Dan, we know how to sequence these; tell us the barcoded-pairs you used on the adaptors for each of your 30libraries and we'll do the rest!"

Also, because I ligated adaptors as opposed to PCR'ed them on (no risk of index-switching on the flow-cell?) I didn't feel the need to clean the libraries as much as I could have. As cleaning a little too stringently would significantly bias my 30-library pool, if you want the sample cleaned more so you get the warm-fuzzies for sequencing, please let me know so I can do that here gently as to affect the sample-to-sample-read-distribution as little as possible.