GC3F-AM-1327 - Snail-2bRAD - 2bRAD

Run Info

Start / End: -

Submitter Info

Lab: Mahon, Andrew (CMU) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
Length Preferred
75

Details

Fragment Size From
50
Fragment Size To
180
Volume Submitted (µL)
250 microliters
Measured Concentration
5.00
Method for Measuring Concentration
Fluorometer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
2bRAD methods using the methods and barcodes of Eli Meyer.

5' end of construct 3' end of construct
(Illumina likes to call this "i5") (Illumina likes to call this "i7")
Name Sequence Name Sequence
HT2 AAGGC TSBC08 ACTTGA
HT3 TGTGT TSBC16 CCGTCC
HT6 GGATG TSBC24 GGTAGC
HT11 CCTGA TSBC35 CATTTT
HT14 CTCGG TSBC39 CTATAC
HT24 AGTGA TSBC48 TCGGCA
HT32 TACCA UTBC50 AAACAC
HT41 TGAAG UTBC85 GCCGCG
TSBC03 TTAGGC
TSBC19 GTGAAA
UTBC54 GATACA
UTBC55 GGTGTG

iLab

iLab State
completed
Lab
Mahon, Andrew (CMU) Lab