GC3F-NW-1412 - Pleuroceridae_Ecoc_Ebell_2bRAD - 2bRAD

Run Info

Start / End: -

Submitter Info

Lab: Whelan, Nathan (US-FWS) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
Length Preferred
75

Details

Volume Submitted (µL)
248
Measured Concentration
3.00
Method for Measuring Concentration
Fluorometer
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
This is my first time submitting a sequencing order for a 2bRAD library prep, but I think everything is correct. I followed the standard 2bRAD protocol, which is the same protocol a colleague of mine, Andy Mahon, has used and sequenced with your facility. So, I do not think custom primers are required.

I measured concentrations of each library by fluorometry and pooled to 3nM. The nM concentration was based off the assumption that the average fragment length is the length of 2bRAD fragment that is cut out of the gel (166 bp).

Please let me know if there are any questions or if anything is not clear in regards to the information under primer kit.

iLab

iLab State
completed
Lab
Whelan, Nathan (US-FWS) Lab