Service
Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
75
Length Preferred
75
Details
Volume Submitted (µL)
248
Measured Concentration
3.00
Method for Measuring Concentration
Fluorometer
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Whelan, Nathan (US-FWS) Lab
I measured concentrations of each library by fluorometry and pooled to 3nM. The nM concentration was based off the assumption that the average fragment length is the length of 2bRAD fragment that is cut out of the gel (166 bp).
Please let me know if there are any questions or if anything is not clear in regards to the information under primer kit.