Flow Cytometry

 

Flow Cytometry

The GC3F houses two flow cytometers: a Sony SH800 cell sorter and an Applied Biosystems Attune Cytometer.  Both instruments are user-operated.  The GC3F provides training on both (email Doug Turnbull: dturnbul@uoregon.edu to schedule a training session), but it is up to you to analyze and sort your own cells.  Both the Sony and Attune are intuitive, user-friendly instruments, so don’t be afraid to get trained and analyze your cells!

Sony SH800

The Sony SH800 is a cell sorter capable of distinguishing up to 6 different fluorescent markers (excited by 4 lasers: red, green, violet, and blue) in a mixed population of cells.  Alignment and calibration are all handled automatically using disposable optical plastic sorting chips that are based on Sony Blu-ray technology from the home consumer electronics realm.

The SH800 can sort cells in to two collection tubes at a time, and is capable of sterile sorting following an approximately 2.5 hour long instrument decontamination procedure.

NOTICE

Beginning on May 1, 2017 the GC3F will begin charging users a small fee to operate the Sony SH800 Cell Sorter:

  • $35 for a sorting chip, either 100µm or 130µm size
  • $40 per hour for instrument usage time, including time needed for startup and shutdown

If requested, an optional 2.5-hour instrument decontamination procedure can also be included (please request well in advance):

  • $200 for GC3F staff to perform the decontamination procedure for you
  • $100 for you to perform the decontamination procedure yourself (requires prior training with GC3F staff)

Users will be billed based on their reservations in the Google Calendar below. Therefore, we are asking that all users now include billing information in their calendar reservations:

  • For on campus users, please list your billing index (and activity code if applicable) in the reservation.
  • For off campus users, please provide in the reservation detailed billing instructions that we can forward along to our Business Services department.

If you end up not using your reservation, or you use the instrument longer or shorter than expected, please delete or update your calendar reservation accordingly. Contact Maggie Weitzman (mweitzm2@uoregon.edu) with questions or to request calendar access. Thank you!

 

 

Applied Biosystems Attune

The Applied Biosystems Attune cytometer is a cytometer (not capable of sorting) that can measure fluorescent markers that are excited by blue or violet lasers.  This instrument is a good alternative to the Sony if sorting is not needed, and the panel of fluorescent markers does not require the 6 detectors of the Sony.