Researchers using the GC3F facility or instruments should acknowledge the facility and personnel in reporting, publications, and presentations.
Publication and Acknowledgement Expectations
GC3F is a critical research resource that provides access to advanced instrumentation, technical expertise, training, and scientific consultation.
Users are expected to acknowledge the facility in all publications, presentations, posters, theses, and other scholarly works that include data generated using facility resources. Acknowledging the facility demonstrates the impact of shared research infrastructure, supports continued institutional and external funding, and helps ensure the long-term sustainability of these resources.
If facility staff make substantial intellectual contributions to a project—including experimental design, development or optimization of methods, complex data analysis, interpretation of results, or manuscript preparation—they should be offered co-authorship in accordance with accepted authorship guidelines and journal policies.
Users are encouraged to notify GC3F upon acceptance of any publication that includes data generated using facility resources and to provide a URL link or PDF of the publication for facility records.
Acknowledging the GC3F Template Language
"The authors acknowledge the GC3F Shared Instrument Facility for providing access to the [Genomics facility/ Flow Cytometry facility / Light Microscopy facility], including instrumentation, technical expertise, training, and scientific consultation that contributed to this work. The [instrument name/model] used in this study was acquired with support from [funding source, grant number, if applicable].''
If multiple services contributed to the work, please list all that are applicable (e.g., Genomics, Flow Cytometry, and Light Microscopy) within the acknowledgment.
Please use the following links as additional guides.
What to Report in Your Methods Section
Microscopy Example
Please contact Adam Fries for specific microscopy related equipment information relevant to your methods.
Z-stacks spanning 20 µm were acquired with a 650-nm step size using a Cytiva DeltaVision Ultra widefield fluorescence microscope equipped with an Olympus UPlanSApo 20×/0.75 NA air objective, a PCO Edge 42Q.GE sCMOS camera (6.5-µm pixel size), and a Lumencor Spectra X light engine. Images were collected in the Blue, Green, and Orange channels using the DeltaVision Blue-Green-Orange-FarRed polychroic filter set. Filter specifications (center wavelength/bandpass, nm) were as follows: Blue (Ex 390/22, Em 435/48), Green (Ex 475/33, Em 525/48), Orange (Ex 542/32, Em 597/45), and Far Red (Ex 632/28, Em 679/34). Exposure times and illumination power measured at the specimen plane were adjusted independently for each channel to maximize dynamic range while avoiding detector saturation (Blue: 100 ms, 5% LED power, 2.2 mW at the specimen plane; Green: 200 ms, 10% LED power, 2.1 mW at the specimen plane; Orange: 150 ms, 5% LED power, 1.3 mW at the specimen plane). Raw image stacks were deconvolved using SoftWoRx v7.2.1 (Cytiva) with the standard Enhanced Ratio algorithm, 10 iterations, and factory-supplied optical transfer functions. The microscope is maintained by the Genomics and Cell Characterization Core Facility at the University of Oregon.
Please use the following links as useful guides:
Flow Cytometry Example
Example to come
Sequencing Example
Example to come
Acknowledging Grant-supported Instrumentation
Grant-Supported Instruments
List to come
Institutionally Supported Instruments
List to come
Donor Supported Instruments
- Cytiva DeltaVision Ultra - Donald E. and Delia B. Baxter Foundation
- Nikon Ti2 with Yokogawa CSU-W1 SoRa Spinning Disk - Murdock Charitable Trust
Impact Metrics
Microscopy: 3,915 hours (2023–2024), 3,979 hours (2024–2025), 4,663 hours (2025–2026)
Publications using the Facility
2019 | 2020 | 2021 | 2022 | 2023 | 2024 | 2025
2025
- Le Bleu, H. K., Kioussi, R. G., Henner, A. L., Lewis, V. M., Stewart, S., Stankunas, K. (2025). Voltage-gated calcium channels generate blastema Ca2+ fluxes restraining zebrafish fin regenerative outgrowth. bioRxiv 2024.08.21.608903.
- Lewis V.M., Fernandez R.A., Horst S.G., Gonzalez C.A., Stankunas K. (2025) Early exercise disrupts a pro-repair extracellular matrix program during zebrafish fin regeneration. bioRxiv 2024.11.15.623835.
2024
- LaFoya, B., Penkert, R. R. & Prehoda, K. E. The cytokinetic midbody mediates asymmetric fate specification at mitotic exit during neural stem cell division. BioRxiv Prepr. Serv. Biol. 2024.08.27.609974 (2024) doi:10.1101/2024.08.27.609974.
- LaFoya, B. & Prehoda, K. E. Membrane oscillations driven by Arp2/3 constrict the intercellular bridge during neural stem cell divisions. BioRxiv Prepr. Serv. Biol. 2024.10.28.620743 (2024) doi:10.1101/2024.10.28.620743.
- LaFoya, B., Welch, S. E. & Prehoda, K. E. Lgl resets Par complex membrane loading at mitotic exit to enable asymmetric neural stem cell division. BioRxiv Prepr. Serv. Biol. 2024.09.29.615680 (2024) doi:10.1101/2024.09.29.615680.
- Narvaez-Ortiz, Heidy Y; Lynch, Michael J; Liu, Su-Ling; Fries, Adam; Nolen, Brad J (2024). Both Las17-binding sites on Arp2/3 complex are important for branching nucleation and assembly of functional endocytic actin networks in S. cerevisiae. PMCID: PMC10944109 PMID: 38367669.
- Jones, K. A., Drummond, M. L., Penkert, R. R. & Prehoda, K. E. Cooperative regulation of C1-domain membrane recruitment polarizes atypical protein kinase C. J. Cell Biol. 222, e202112143 (2023).
- LaFoya, B. & Prehoda, K. E. Consumption of a polarized membrane reservoir drives asymmetric membrane expansion during the unequal divisions of neural stem cells. Dev. Cell 58, 993-1003.e3 (2023).
- Lewis, V.M., Le Bleu, H.K., Henner, A.L., Markovic, H., Robbins, A.E., Stewart, S., Stankunas, K. (2023). Insulin-like growth factor receptor / mTOR signaling elevates global translation to accelerate zebrafish fin regenerative outgrowth. Developmental Biology 10.1016/j.ydbio.2023.05.008. PMC10866574.
- Robbins, A.E., Horst, S.G., Lewis, V.M., Stewart, S., Stankunas, K. (2023). The Fraser complex interconnects tissue layers to support basal epidermis and osteoblast integrated morphogenesis underlying fin skeletal patterning. bioRxiv 2023.07.08.548238. PMC10350090.
2022
- Desvignes T., Robbins A.E., Carey A.Z., Bailon-Zambrano R., Nichols J.T., Postlethwait J.H., Stankunas K. (2022). Coordinated patterning of zebrafish caudal fin symmetry by a central and two peripheral organizers. Dev Dyn., 251(8):1306-1321. PMC9357109.
- Ding, Bojian; Narvaez-Ortiz, Heidy Y; Singh, Yuvraj; Hocky, Glen M; Chowdhury, Saikat; Nolen, Brad J (2022). Structure of Arp2/3 complex at a branched actin filament junction resolved by single-particle cryo-electron microscopy. PMCID: PMC9295785 PMID: 35622886.
- Liu, Su-Ling; Narvaez-Ortiz, Heidy Y; Miner, Matt; Kiemel, Jack; Oberhelman, Nicholas; Watt, April; Wagner, Andrew R; Luan, Qing; Helgeson, Luke A; Nolen, Brad J (2022). Analysis of functional surfaces on the actin nucleation promoting factor Dip1 required for Arp2/3 complex activation and endocytic actin network assembly. PMCID: PMC9168731 PMID: 35533729.
- Narvaez-Ortiz, Heidy Y; Nolen, Brad J (2022). Unconcerted conformational changes in Arp2/3 complex integrate multiple activating signals to assemble functional actin networks. PMCID: PMC8930562 NIHMSID: NIHMS1775125 PMID: 35090589.
2021
- Braunstein, J.A., Robbins, A.E., Stewart, S, Stankunas, K. (2021). Basal epidermis collective migration and local Sonic hedgehog signaling promote skeletal branching morphogenesis in zebrafish fins. Developmental Biology 477, 177-190. PMC10802891.
- LaFoya, B. & Prehoda, K. E. Actin-dependent membrane polarization reveals the mechanical nature of the neuroblast polarity cycle. Cell Rep. 35, 109146 (2021).
- Oon, C. H. & Prehoda, K. E. Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle. eLife 10, e66574 (2021).
- Stewart, S., Le Bleu, H. K., Yette, G. A., Henner, A. L., Braunstein, J. A., Stankunas, K. (2021). longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth. Development 148, dev199384. PMC8217709.
2020
- Balzer, Connor J; James, Michael L; Narvaez-Ortiz, Heidy Y; Helgeson, Luke A; Sirotkin, Vladimir; Nolen, Brad J (2020) Synergy between Wsp1 and Dip1 may initiate assembly of endocytic actin networks. PMCID: PMC7707826 PMID: 33179595.
2019
- Oon, C. H. & Prehoda, K. E. Asymmetric recruitment and actin-dependent cortical flows drive the neuroblast polarity cycle. eLife 8, e45815 (2019).
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