Illumina NovaSeq now available in GC3F!
Thanks to a generous award from the Murdock Charitable Trust, GC3F is now offering Illumina sequencing services on a NovaSeq 6000 sequencer. The NovaSeq will be taking the place of our workhorse HiSeq 4000, and offerings the following advantages over the older platform:
- Multiple output modes from 400M up to 2500M read pairs per lane
- Lower cost per Gbp than HiSeq 4000, even in the lowest NovaSeq output mode (SP)
- Lowest cost per Gbp of any available sequencing platform in high output (S4) mode
- Fewer lanes per flowcell = less lead time waiting for lane submissions from other users needed to launch runs
- Full compatibility with all Illumina library types
Log in to iLab to submit a sample to run on the NovaSeq!
Pricing info for single-lane NovaSeq sequencing
Read Pairs per lane**
|S4 300 Cycle||$4500||$5625||PE 2x150||2-2.5 billion+|
|S4 35 Cycle||$3300||$4163||SR 53*, PE 2x32*||2-2.5 billion+|
|S1 300 Cycle||$3300||$4163||PE 2x150||650-800 million+|
|S1 100 Cycle||$2490||$3113||SR 118*, PE 2x59* 10X scRNA||650-800 million+|
|SP 500 Cycle||$2700||$3375||PE 2x250||325-400 million+|
|SP 300 Cycle||$1980||$2475||PE 2x150||325-400 million+|
|SP 100 Cycle||$1440||$1800||SR 118*, PE 2x59* 10X scRNA||325-400 million+|
*All NovaSeq kits contain sufficient reagent to sequence an additional 38nt beyond the specified cycle number. These can be used for 10X cell barcodes, long dual index reads, or extended read length for the library inserts
**These are the official Illumina output specs and are conservative. Real world performance will often exceed these read numbers
Notice: HiSeq 4000 Service ending June 1st.
After a years of very productive use, our HiSeq 4000 will be going offline at the end of May. We will make sure that all existing submissions are either loaded on HiSeq runs before then, or sequenced on our new NovaSeq 6000. If you absolutely must have HiSeq data (additional replicates for a long running project, etc.), please contact us and we will do our best to accomodate your request.
Please note that all of the NovaSeq run modes now provide a lower cost per GB of data than the HiSeq 4000. This includes NovaSeq SP lanes, which are roughly equivalent in output to HiSeq 4000 lanes. In addition, the NovaSeq has proven to be fully compatible with every library type we have tried (including RAD-seq), so we are confident that making the switch to the new platform will benefit all of our existing HiSeq users!
GC3F ACCESS DURING COVID-19 PANDEMIC
We are currently operating according to policies described in our approved Stage 1 Recovery Plan.
A summary of these policies is posted up around the core, as well as HERE.
FAQs ON GC3F's OPERATIONAL STATUS DURING COVID-19 PANDEMIC
CLICK HERE to get answers to our most frequently asked COVID-19 operational status questions.
NEW POLICY ON DATA RETENTION
We have had an increasing number of requests for the retrieval of old sequencing data from our archive. While we are always happy to help whenever we can, a couple of issues have arisen surrounding this. CLICK HERE to review our new policy on data retention & retrieval.
The Genomics and Cell Characterization Core Facility (GC3F) supports scientific research at the University of Oregon by making a broad array of high end specialized instrumentation accessible to UO researchers for genomics, flow cytometry, and light microscopy applications. We also offer in-house sequencing sample prep services, for both internal and external users.
Please click the tabs above to learn about the services provided by the GC3F!