GC3F-RV-60 - BetterAir-16S - 16S & ITS

Run Info

Start / End: -

Submitter Info

Lab: Van Den Wymelenberg, Kevin (UOREGON) Lab

Service

Service Requested
MiSeq v2
Read Type
Paired-End
Length Preferred
250

Details

Fragment Size From
250
Fragment Size To
350
Volume Submitted (µL)
30
Measured Concentration
20.00
Method for Measuring Concentration
Other
Concentration units
nM
Multiplexed?
NO: This is a single library with no barcodes
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
This is the sequencing request for the ITS and 16S preps in the other request. I think we can multiplex the two markers and sequence them together in the same run, right? I'm open to other alternatives if that doesn't sound like a good idea for some reason or other.

I don't know the answers to several of these questions because the library prep isn't done yet, and because you're doing it. But they're required, so... I just sort of filled in something reasonable sounding?

We'll try to get you the DNAs before the end of the month. It might be nice to sit down and talk about the experiment in general, because (we talked about this a little bit), we're also doing some qPCRs on the PMA and non-PMA treated DNAs to look at number of 16S copies in viable cells vs. total, and maybe we should do that before the library preps? I'd just sort of like your advice on the order of operations in general for this experiment.

iLab

iLab State
completed
Lab
Van Den Wymelenberg, Kevin (UOREGON) Lab