Service
Service Requested
NextSeq 500 (High Output)
Read Type
Single
Length Preferred
150
Details
Volume Submitted (µL)
30
Measured Concentration
2.00
Method for Measuring Concentration
Fluorometer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Miller, Adam (Oregon) Lab
Read 1 = 26 nuc (10X barcode + UMI)
Read 2 = 133 nuc (cDNA insert)
Index 1 = 8 nuc (sample index)
TOTAL = 167 nuc (max for NextSeq HO 150 kit)