GC3F-EM-273 - Cat2018 - mixed 2bRAD ad TagSeq

Run Info

Start / End: -

Submitter Info

Lab: Meyer, Eli (OSU) Lab

Service

Service Requested
NextSeq 500 (High Output)
Read Type
Single
Length Preferred
75

Details

Fragment Size From
166
Fragment Size To
350
Volume Submitted (µL)
20
Measured Concentration
25.00
Method for Measuring Concentration
Fluorometer
Primer Kit
Custom
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
This pool contains a mixture of 2bRAD libraries for genotyping and Tag-Seq libraries for gene expression analysis. As such, the molecular weight distribution may look odd. There should be a peak around ~170 and a smear ranging from ~250-350. But please do not delay sequencing of the sample based on any BioAnalyzer results. We're satisfied with the libraries and ready to accept the risk of imperfect yields etc.

The data are for a pair of hands-on workshops we are running so our main priority is getting data as soon as possible, rather than delaying to e.g re-purify or re-size select the pool.

The libraries are DUAL INDEXED and do need to be demultiplexed.

One of the students who prepared libraries has not yet provided me the barcode information. I have filled in NNNNNN for those barcodes in the spreadsheet above but will update those within 24 hours. I just wanted to submit the sample asap so you could get it into the system.

iLab

iLab State
completed
Lab
Meyer, Eli (OSU) Lab