Service
Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
150
Length Preferred
150
Details
Fragment Size From
350
Fragment Size To
450
Volume Submitted (µL)
100
Measured Concentration
93.50
Method for Measuring Concentration
qPCR
Primer Kit
Custom
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Sonsthagen, Sarah (USGS) Lab
These are ddRAD libraries with dual indices (6bp) with TruSeq chemistry adapters. The index sequence orientation is based on what I would request with a MiSeq Reporter. Please let me know if the i5 sequences need to be reverse complemented and will send an updated spreadsheet.