GC3F-ER-1323 - Albo8&9 - ddRAD

Run Info

Start / End: -

Submitter Info

Lab: Burford Reiskind, Martha (NCSU) Lab

Service

Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
100
150
Length Preferred
100

Details

Fragment Size From
200
Fragment Size To
700
Volume Submitted (µL)
50
Measured Concentration
20.00
Method for Measuring Concentration
Bioanalyzer
Primer Kit
Custom
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
Yes

Sample

Expected Date Sample will be Submitted

Payment

Comments
We designed 48 unique variable-length (5-10bp) barcodes for the SphI cut side (P1 adapter) and a fixed-length y-adapter for the MluCI cut site (P2 adapters). We also built two indices into the reverse PCR primer allowing us to multiplex two groups of 48 into one sequencing lane (for a maximum number 96 individuals per lane). We added a custom primer (the spike, sequence above) to our sequence-ready library to pick up our custom indices within the y-adapter, allowing us to successfully de-index libraries of 48 individuals from the Illumina platform (see Supporting information).

Please contact Dr. Martha Burford Reiskind (mbreiski@ncsu.edu) with any questions or for additional details.

iLab

iLab Service ID
GC3F-ER-1323 - Albo8&9 - ddRAD
iLab State
completed
Date Request Submitted
Lab
Burford Reiskind, Martha (NCSU) Lab