Service
Service Requested
HiSeq 4000
Read Type
Single
Length Allowed
100
150
Length Preferred
100
Details
Fragment Size From
200
Fragment Size To
700
Volume Submitted (µL)
50
Measured Concentration
20.00
Method for Measuring Concentration
Bioanalyzer
Primer Kit
Custom
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
Yes
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Pfennig, David (UNC-CH) Lab
and a fixed-length y-adapter for the MluCI cut site (P2 adapters). We also built two indices into the
reverse PCR primer allowing us to multiplex two groups of 48 into one sequencing lane (for a
maximum number 96 individuals per lane). We added a custom primer (the spike, sequence above)
to our sequence-ready library to pick up our custom indices within the y-adapter, allowing us to
successfully de-index libraries of 48 individuals from the Illumina platform (see Supporting
information).
These libraries should be processed identically to order CF-3522126-1 submitted by Emily Reed, Burford
Reiskind Lab (Albo8&9) and sample (2478891) submitted by Nicholas Levis (NL1.1 & 1.2).
Please contact Dr. Martha Burford Reiskind (mbreiski@ncsu.edu) with any questions or for
additional details.