Service
Service Requested
MiSeq Nano
Read Type
Single
Length Preferred
150
Details
Fragment Size From
250
Fragment Size To
300
Volume Submitted (µL)
25
Measured Concentration
19.00
Method for Measuring Concentration
NanoDrop/Spectrophotometer
Primer Kit
Illumina Nextera kit for DNA (96 indices)
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (but demultiplexing is NOT requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
Comments
We are trying to compare sequencing of an identical sample with and without PhiX spike in. We would like only the minimal PhiX spike in one of the lanes (0.5%), and a 25% PhiX spike in the second sequencing lane. This is following up on a sequencing run submitted by Devin Bendixen around February or March of 2018, so we would like to keep the chemistry and parameters as close as possible to that sequencing run. Please don't hesitate to contact me with any questions. Thanks!
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Hayden, Eric (BSU) Lab