GC3F-SP-2412 - OmyKLAR_Run1 - GT-seq

Run Info

Start / End: -

Submitter Info

Lab: O'Malley, Kathleen (OSU) Lab

Service

Service Requested
NextSeq 500 (High Output)
Read Type
Single
Length Preferred
75

Details

Fragment Size From
200
Fragment Size To
200
Volume Submitted (µL)
30
Measured Concentration
15.00
Method for Measuring Concentration
qPCR
Primer Kit
Custom
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
Yes

Sample

Expected Date Sample will be Submitted

Payment

Comments
Please see the document attached here for more details for preparing the custom primer blends and spiking in read primers. Note: custom read primers are to be diluted to 10 uM and spiked into the appropriate positions in the reagent cartridge described in the document. The custom indexing primers are to be pooled and used as a custom blend. Please contact me directly at 856-298-8134 if you need any clarification.

Please use 10% Phi-X spike in for this library.

A comment about the fragment sizes- listed is the median fragment sizes from the BioAnalyzer. Please let me know if you have any questions or concerns.

iLab

iLab Service ID
GC3F-SP-2412 - OmyKLAR_Run1 - GT-seq
iLab State
completed
Date Request Submitted
Lab
O'Malley, Kathleen (OSU) Lab