GC3F-AS-2500 - Doe NB 7-1 10X Run #1 - 10x SC v3 mRNA-Seq

Run Info

Start / End: -

Submitter Info

Lab: Doe, Christopher (UOREGON) Lab

Service

Service Requested
NextSeq 500 (High Output)
Read Type
Single
Length Preferred
150

Details

Volume Submitted (µL)
N/A
Measured Concentration
0.00
Method for Measuring Concentration
Fragment Analyzer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No

Sample

Expected Date Sample will be Submitted

Payment

Comments
not sure if filled out exactly right, will confirm submission with Maggie

(MW 12/10/19) Custom read split for SC v3 chemistry on HO 150 cyc kit (comes with 167 total cycles):

Read 1 = 28 nt (10X barcode @ 16nt + UMI @ 12nt)
Read 2 = 131 nt (cDNA insert)
Index 1 = 8 nt (sample index)

iLab

iLab Service ID
GC3F-AS-2500 - Doe NB 7-1 10X Run #1 - 10x SC v3 mRNA-Seq
iLab State
completed
Date Request Submitted
Lab
Doe, Christopher (UOREGON) Lab