Service
Service Requested
MiSeq v3
Read Type
Paired-End
Length Preferred
300
Details
Volume Submitted (µL)
30
Method for Measuring Concentration
Fluorometer
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
Yes
Sample
Expected Date Sample will be Submitted
Payment
Comments
Above I was not sure what to select - e.g., no this is a single library with no barcodes. I am sending 122 samples of gDNA to be barcoded and pooled for MiSeq using ITS1f and ITS2. I have submitted this sample prep request separately with additional information and spreadsheet. I have requested to amplify the gDNA samples using the ITS1f and ITS2 barcodes you have on hand. Please contact me if you have further questions.
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Vogt, Daniel (UW) Lab
Admin
Date Sample Received