Service
Service Requested
NextSeq 500 (High Output)
Read Type
Paired-End
Length Preferred
75
Details
Volume Submitted (µL)
from core
Method for Measuring Concentration
Other
Primer Kit
Illumina TruSeq kit for RNA (24 indices)
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Single indexed at Index 1 (i7 side)
Custom primers requested?
No
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Doe, Christopher (UOREGON) Lab
Admin
Date Sample Received
Hi Maggie,
I really wasn't sure about a lot of this information since I didn't prep the samples. For example, I didn't know if the 4 samples I submitted were barcoded in a way that they could be sequenced on a single lane, or if they need individual lanes. That obviously has a huge impact on the price. If reading depth goes down by pooling to a single lane, we might consider splitting the samples over 2 lanes. I also don't know what the index sequences are- I assume they come from the NextGem kit? If something doesn't look right and I need to modify, please let me know!
Thanks!
Sarah