Service
Service Requested
NextSeq 500 (High Output)
Read Type
Single
Length Preferred
75
Details
Fragment Size From
180
Fragment Size To
800
Volume Submitted (µL)
30
Measured Concentration
13.00
Method for Measuring Concentration
qPCR
Concentration units
nM
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
Yes
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
O'Malley, Kathleen (OSU) Lab
Admin
Date Sample Received
Attached methods for loading custom primers for run. We will send all custom primers in 30 uL aliquots (cRead1, cRead2, cIndex1 and cIndex2) at 100 uM. If these could be kept for future runs, that would be great as well.
Note for Run Setup:
When entering run parameters, check the boxes under Index 1 and Index 2 to indicate custom primers. Do not check the boxes under Read 1 or Read 2.