We are having the pooled sample prepared by the GC3F, so I do not know the volume and concentration of what will be submitted for sequencing, or when it will be submitted/ready for sequencing. In the past we have done this ourselves and the multiplexed molarity was 10 nM, so that is what I put in the form.

I can't access specific info from previous orders, so I had to make my best guess as to whether we usually request demultiplexing, and which side the index is on. I had difficulty finding information on this, but I *think* the index is at the i7 side - it will most definitely be the same as for all of our previous orders. Since this is my first time submitting samples, please let me know if anything looks amiss or deviates from our usual submissions. Thank you!