GC3F-CG-5259 (#5267)

Submitter Info

  • User: Claire Goodfellow
  • Lab: Ting, Nelson (UOREGON) Lab

Service

Service Requested: 
MiSeq v2
Read Type: 
Paired-End
Length Preferred: 
250

Details

Volume Submitted (µL): 
Pending
Measured Concentration: 
Pending
Method for Measuring Concentration: 
qPCR
Primer Kit: 
Custom
ng/µL
Multiplexed?: 
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme: 
Dual indexed (both i5 and i7 sides)
Custom primers requested?: 
No

Sample

Expected Date Sample will be Submitted: 
Wednesday, November 10, 2021

Payment

Comments: 
Custom sequencing request: Please use the information described below "All STR amplicons were sequenced in one direction using v2 chemistry (500 cycle kits) without fragmentation. This increased the length of the STR loci that could be analyzed to ~400 bp (instead of 2 x 250 paired-end reads). Although 500 cycles are the theoretical maximum of the sequencing kit, we observed diminishing data quality between 350 and 400 cycles. We thus selected 375 forward and 51 reverse read cycles, using only the forward reads for analysis to preclude alignment artifacts of pairing reads in the repeat regions (the reverse reads were only used for MiSeq quality control)." Samples have been submitted for pooling under request name GC3F-CG-5253.

iLab

iLab Service ID: 
iLab State: 
completed
Name: 
Claire Goodfellow
Lab: 
Ting, Nelson (UOREGON) Lab

Admin

Date Sample Received: 
Wednesday, November 17, 2021