Service
Service Requested
MiSeq v2
Read Type
Paired-End
Length Preferred
150
Details
Volume Submitted (µL)
40
Measured Concentration
0.801
Method for Measuring Concentration
Fluorometer
Primer Kit
Custom
Concentration units
ng/µL
Multiplexed?
YES: Index reads are required (and demultiplexing IS requested)
Indexing scheme
Dual indexed (both i5 and i7 sides)
Custom primers requested?
Yes
Sample
Expected Date Sample will be Submitted
Payment
iLab
iLab Service ID
iLab State
completed
Date Request Submitted
Lab
Hauser, Lorenz (UW) Lab
Admin
Date Sample Received
Read1 = can use the Illumina primers in the cartridge* but would see better results with a custom primer 5'-CGACAGGTTCAGAGTTCTACAGTCCGACGATC-3' If your library is low diversity and you also need to sequence PhiX, you would spike this into the Illumina well.
I'm sending two libraries and would like to sequence both on one lane. Could you do QC on both and combine them together? They each contain 96 unique dual barcoded individuals, and they can be combined with no issues. Thanks!