Custom run request: extend forward read from 300 to 375 forward read cycles, to adapt run to follow Barbian et al. excerpted below:

"All STR amplicons were sequenced in one direction using v2 chemistry (500 cycle kits) without fragmentation. This increased the length of the STR loci that could be analyzed to ~400 bp (instead of 2 x 250 paired-end reads). Although 500 cycles are the theoretical maximum of the sequencing kit, we observed diminishing data quality between 350 and 400 cycles. We thus selected 375 forward and 51 reverse read cycles, using only the forward reads for analysis to preclude alignment artifacts of pairing reads in the repeat regions (the reverse reads were only used for MiSeq quality control)."