Sample Prep Services

The GC3F offers full-service sample preps for many popular sequencing library types, including stranded mRNA-Seq, whole genome shotgun, 16S amplicon, 10X whole genome and single cell mRNA-Seq, PacBio, and many others -- including custom projects by request! Please contact us to inquire.

All library prep services include numerous quality control (QC) steps, including but not limited to:

  • Initial quantifcation and fragment analysis of your input samples
  • Barcoding of multiple libraries using uniquely dual indexed adapters
  • Size selection/dilution/concentration
  • qPCR quantification and fragment analysis of each barcoded sub-library
  • Final multiplexing based on qPCR results

Contact Maggie Weitzman (mweitzm2@uoregon.edu) for details on sample drop off, etc.

 

SAMPLE PREP TYPE

EXTERNAL PRICE *

INTERNAL PRICE *

RECOMMENDED INPUTS

Stranded mRNA-Seq $255 per sample $155 per sample 100 to 4,000 ng of high quality total RNA in < 50 µL of RNase-free water or buffer
Stranded RNA-Seq (does not include mRNA capture)  $255 per sample $155 per sample 1 to 100 ng of purified total, rRNA-depleted, or poly(A)-enriched RNA in < 10 µL of RNase-free water or buffer
Whole genome shotgun (WGS) or metagenome shotgun  $230 per sample $140 per sample 1 to 1,000 ng of pure gDNA in < 50 µL of EDTA-free buffer
DNA from any experiment that is >1kb (requires shearing/size selection)  $230 per sample $140 per sample 1 to 1,000 ng of pure DNA in < 50 µL of EDTA-free buffer
DNA from any experiment that is <1kb (has already been sheared/size selected) $200 per sample $110 per sample 1 to 1,000 ng of pure DNA in < 50 µL of EDTA-free buffer
16S (bacterial community profiling), v4 region $1,440 per plate of 96 samples $900 per plate of 96 samples Any amount of pure DNA (more = better!) in at least 30 µL volume
16S (bacterial community profiling), v3-v4 region $1,440 per plate of 96 samples $900 per plate of 96 samples Any amount of pure DNA (more = better!) in at least 30 µL volume
BluePippin size selection + QC/concentration $75 per sample $75 per sample Varies - contact us for details
10X Single Cell mRNA-Seq Not available for external customers $1,555 per sample 100 to 2,000 viable cells/µL (washed & filtered single cell suspension) in 10-50 µL volume
10X Whole Genome Linked Reads  $1,050 per sample $700 per sample Varies - contact us for details
PacBio Sequencing Library $675 per sample $450 per sample At least 5 µL of high quality purified DNA (contact us if you have less than this)
High molecular weight DNA extraction  + QC/concentration $25 per sample $25 per sample Varies - contact us for details
Low sample biomass DNA extraction + QC/concentration Varies - contact us for details Varies - contact us for details Varies - contact us for details
RNA extraction + QC/concentration Varies - contact us for details Varies - contact us for details Varies - contact us for details
DNase treatment of RNA + QC/concentration $20 per sample $20 per sample Varies - contact us for details
QC/multiplexing of completed libraries $12.50 per sample $12.50 per sample Any completed libraries
QC/multiplexing of completed libraries + size selection/concentration  $25 per sample $25 per sample Any completed libraries
Other/custom Varies - contact us for details Varies - contact us for details Varies - contact us for details

* Final prices may differ slightly depending on project complexity, extra steps needed, etc.