Microscopy Instruments

The light microscopy center within the GC3F offers training and support for the following equipment:

Black and white image of a Leica SPE laser.

Leica SPE Laser Scanning 

Confocal and Widefield

  • 405nm, 488nm, 561nm, 633nm laser excitation lines, and DIC illumination
  • DAPI, FITC, TRITC equivalent for widefield imaging
  • Motorized stage, upright body
  • 5x, 10x, 25x water dipping, 40x oil, 40x water dipping, 63x oil objectives
  • Acquisition software is LAS X on Windows 10

Black and white image of a microscope.

Cytiva DeltaVision Ultra 

Widefield with Deconvolution

  • LED excitation lines of 405nm, 458nm, 488nm, 514nm, 555nm, 594nm, 633nm
  • 20x LWD, 20x, 60x oil, 100x oil objectives
  • Motorized stage, pco.edge sCMOS camera, inverted body
  • Acquisition software is Acquire Ultra on Linux CentOS 7

Black and white image of a microscope.

Zeiss LSM 880 

With Elyra Structured Illumination and Airy Scanning

  • 405nm, 458nm, 488nm, 514nm, 561nm, 633nm laser excitation lines
  • 10x, 20x, 40x water immersion, 40x oil, 63x oil objectives
  • Motorized stage, light-tight enclosure, inverted body
  • Acquisition Software is Zen Black on Windows 7

Black and white image of a microscope.

Nikon CSU-W1 SoRa Spinning Disk

  • Spinning Disk excitation lines are 405nm, 488nm, 561nm, 633nm, and DIC illumination
  • TIRF imaging with excitation lines of 488nm, 561nm, and 633nm
  • Digitial Multi-Mirror Device and Lumencor Spectra X for photo-stimulation with LED stimulation lines of 390nm, 440nm, 488nm, 561nm, 594nm, 633nm, 740nm
  • 405nm photobleaching laser line
  • 4x, 10x, 20x, 40x water immersion, 60x water immersion, 60x oil TIRF objectives
  • Motorized stage, dual Prime BSI sCMOS cameras for optional simultaneous green/red imaging, inverted body
  • Acquisition sofware is Nikon Elements + JOBs on Windows 10

Black and white image of a scanner.

Amersham Typhoon Scanner 

  • fluorescent imaging with multiple lasers (Cy2, Cy3, Cy5)
  • imaging of storage phosphor screens
  • densitometric imaging

Black and white image of a microscope.

Zeiss LSM 710 

With Elyra Structured Illumination and Airy Scanning

This is located in the Knight Campus.

  • 405nm, 458nm, 488nm, 514nm, 561nm, 633nm laser excitation lines
  • 20x LWD, 63x oil, 100x oil objectives
  • Motorized stage, light-tight enclosure, inverted body
  • Acquisition Software is Zen Black on Windows 7

Black and white image of a temperature controller.

Cherry Temp System 

  • Enables temperature shifts from 5-45 celsius degrees in 10 seconds with a 0.1 degree error.

Black and white image of an environmental chamber.

Tokai Hit Environmental Chamber 

  • Portable stage top incubation system

Black and white image of a Leica SPE laser.

Custom-built Light Sheet Microscope 

If you have a project that you think may benefit from light sheet imaging, please contact Adam Fries at afries2@uoregon.edu.


Workstations

GC3F has four analysis workstations and the following software.

  • Imaris 9.9 with Cell and Filaments Modules
  • Imaris 9.9 Converter and Stitcher
  • Prism8
  • Matlab 2018a
  • Adobe Illustrator and Photoshop
  • Microsoft Office
  • Zen Blue/Black
  • Python3
  • FIJI

Contact Adam Fries for more information regarding instruments, training, or analysis, or visit the office in Klamath 270D.

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