Size Selection

BluePippin  

Automated size selection of nucleic acid fragments anywhere between 50 and 50,000 bp in size. Easier, safer, and more reproducible than traditional agarose gel extraction protocols involving a UV illuminator and a razor blade. We sell cassettes to UO users at-cost as needed.   

BluePippin can be reserved in iLab.


Omega Beads  

Omega Bio-Tek's TotalPure NGS Mag-Bind Beads can be used to purify and size-select both DNA.  UO researchers can purchase aliquots of these beads from the GC3F for $7 per mL. We have run a full series of tests to characterize their DNA size selection properties at varying ratios for removing either large or small fragments, a table of the results is available below (courtesy of Maggie Weitzman). 

File A table of bead to sample ratios for removing small or large fragments.

 

Omega Beads can be purchased through the iLab request system.