Whole-genome Sequencing

Mini Echo DNA Prep

A very low-cost Nextera library prep miniaturized using our Echo liquid handler. Suitable for small to medium genomes and DNA of moderate quality or better (>50% of DNA fragments over 1000bp). Requires >25ng of DNA per sample suspended in 25-50ul of water or buffer that is free of EDTA.

Shotgun Prep

Whole-genome sequencing using enzymatic fragmentation. Our option for large genomes and DNA of any quality. A PCR-free workflow is available. Requires >50ng of DNA per sample suspended in 25-50ul of water or low-EDTA buffer.

RNA

Stranded RNA-seq

Stranded, high-complexity RNA-seq libraries for RNA of any quality. Available with or without bead-based mRNA capture. Requires >5ng of total or enriched RNA suspended in 25-50ul of RNase-free water. 

Small RNA-seq

RNA sequencing for miRNA and other small non-coding RNAs. Requires >5ng of total or enriched RNA suspended in 25ul of RNase-free water. 

16S

Amplification of the 16S region by PCR. We offer barcoded primers, allowing up to 768 samples to be pooled for sequencing.

Hi-C

Phase Genomics’ proximity ligation protocol, typically used alongside long-read data to reach chromosomal-level assemblies. Requires >50mg of flash-frozen tissue. Other preservation methods, such as RNAlater or ethanol, are acceptable but may impact library yield or complexity. 

Single-cell 

3' Gene Expression

3' gene expression at the single-cell level. We offer GEX from 10X, Parse, Scale, or ddSeq. Reach out to discuss which platform best fits your project.

ATAC-seq

Chromosomal accessibility information at the single-cell level. We offer ATAC-seq from 10X and ddSeq.

Multiome

Combined 3' GEX and ATAC seq data at the single-cell level. 10X is the only platform for this protocol at this time.

If you don’t see the right prep or aren’t sure what you need, email us at genomics@uoregon.edu.