Quality Control

Fragment Analysis

Automated capillary electrophoresis for measuring fragment sizes in a sample. NGS runs are for fragments 1bp-6000bp, LF quantifies fragments 50bp-165,000bp, and RNA is for RNA of any length.


Purification

Standard Bead Cleanup

Magnetic bead-based purification of samples in 1.5 ml tubes or 96-well plates. If you would prefer to run your own bead purification, we also offer Omega beads for purchase by UO researchers through iLab.  

DNase or RNase Treatment

Enzymatic removal of contaminating DNA or RNA from user-submitted sample. 


Size Selection

Sage BluePippin

Automated size selection of nucleic acid fragments anywhere between 50 and 50,000 bp in size. 

Bead-based Size Selection

Remove small fragments (up to 3 kb) from DNA extractions or completed libraries. If you would prefer to run your own bead size-selection, we also offer Omega beads for purchase by UO researchers through iLab.


Multiplexing of Illumina libraries

qPCR quantification followed by equimolar pooling for sequencing. Only required when submitting more than one tube for multiplexing on an Illumina lane.


Normalization

High-throughput normalization of user-submitted extractions to a desired concentration on our Hamilton Vantage robot.


Speedvac Concentration

Concentrate up to 4 plates of samples on our Centrifugal evaporator. The Speedvac is also available for reservation through our instrument reservation system. 


 

If you don’t see the right prep or aren’t sure what you need, email us at genomics@uoregon.edu