DNA

Standard

Manual extraction of DNA for smaller projects. 

High Molecular Weight 

Extraction of high molecular weight DNA for long-read applications. We use a variety of different protocols depending on the tissue type, including NanoBind Pan DNA from PacBio and Qiagen Genomic tips. 

HMW Tissue Submission Requirements 

Freshly-harvested tissues should be flash-frozen in liquid nitrogen and stored at -80°C. Other preservation methods, such as RNAlater, typically result in poor sequencing performance. Amount of tissue required: 

• For plant extractions, provide at least 3-5g of flash-frozen young leaves  
• For animal extractions, provide one of the following:
  • >1g of low-DNA-content tissue (muscle, skin)
  • >100mg of high-DNA-content tissue (liver, spleen)
  • >50 ul of blood containing nucleated RBC  
  • >500 ul of blood containing non-nucleated RBC  
• For cultured bacteria, >20M cells 

For other extractions, mass required will vary substantially depending on your organism, tissue, and sequencing application. Please email genomics@uoregon.edu to discuss. 

RNA 

Extraction of RNA using Zymo Direct-Zol. We recommend flash-freezing or storing in a stabilizer such as Trizol or RNAlater.  

If you don’t see the right prep or aren’t sure what you need, email us at genomics@uoregon.edu.