Liquid Handling Instruments

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GC3F ACCESS DURING COVID-19 PANDEMIC

We are currently operating according to policies described in our approved Stage 1 Recovery Plan.

A summary of these policies is posted up around the core, as well as HERE.

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FAQs ON GC3F's OPERATIONAL STATUS DURING COVID-19 PANDEMIC

CLICK HERE to get answers to our most frequently asked COVID-19 operational status questions.

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NEW POLICY ON DATA RETENTION

We have had an increasing number of requests for the retrieval of old sequencing data from our archive. While we are always happy to help whenever we can, a couple of issues have arisen surrounding this. CLICK HERE to review our new policy on data retention & retrieval.

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The GC3F houses modern liquid handling instruments that are available to the UO community to help reduce your pipetting burden and streamline your sample prep process!

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Rainin Liquidator 96: High Throughput Pipetting

The GC3F houses two Rainin Liquidator 96 instruments that operate at 20 µL and 200 µL max volumes. These instruments work just like manual hand-held pipettes (2-stage plunger mechanism) and allow consistent pipetting of reagents into all wells of a 96-well plate in one fluid motion! For this reason, some researchers like to call them "plate stampers."

The Liquidators use specialized sterilized PCR-clean filter tips, which we stock in house and sell to users at cost.

Contact Maggie Weitzman (mweitzm2@uoregon.edu) for training on the Liquidators prior to use.

CLICK HERE to schedule a reservation on the the Liquidators! (Note, you will need to register for an iLab account.)

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Eppendorf EpMotion 5075 TMX: Liquid Handling Robotics

The GC3F houses an Eppendorf EpMotion 5075 TMX liquid handing robot, which has a 13-position work table capable of accepting a diverse array of multi-well sample plates.  We also have adapters for working with standard microtubes and large reservoir troughs.
 
 
The EpMotion also has a “thermo-mix” position on the worktable that is capable of vortex mixing as well as heating / cooling samples in a 96-well plate.  This allows some enzymatic reactions to be conducted on the robot worktable for automated, high throughput sample processing.

PCR-clean filter tips for the robot are available in the core (in 50 µL and 1000 µL sizes) and can be purchased from the GC3F at cost.  The hourly fee for robot usage is $25 per hour. 

Contact Maggie Weitzman (mweitzm2@uoregon.edu) for training on the EpMotion prior to use.

CLICK HERE to schedule a reservation on the epMotion! (Note, you will need to register for an iLab account.)