__________________________________________________________
Summary of Sample Prep Services
GC3F offers full-service sample preps for many popular sequencing library types, including custom projects by request! See the list of library and template prep services below. If the right prep for your project is not listed, reach out to us at genomics@uoregon.edu to discuss a custom project.
Internal pricing is available for University of Oregon, Oregon State University, Oregon Health and Science University, and Portland State University. External pricing applies for all other requesters.
To submit a request for Sample Prep service, please register for an iLab account and then click "Request Services" from the top menu bar in iLab. It is important that your request has been submitted in iLab before you provide the physical samples to us.
CLICK HERE to submit a Sample Prep request!
__________________________________________________________
Shipping Instructions for Sample Prep Projects
Please deliver samples 288 Klamath Hall if you are an on-campus user. For off campus users, please mail samples frozen on dry ice in a well-protected vessel to the following address:
University of Oregon/GC3F
1318 Franklin Blvd
Room 273 Onyx Bridge
Eugene, OR 97403
Please ensure that your package will arrive at UO Mon-Fri. UO cannot accept deliveries on weekends or federal holidays.
Please ensure that samples are extremely well sealed before shipping. Adhesive foil seals should be buffed on for extra security (video demonstration).
__________________________________________________________
|
Library Prep Services |
Internal Price |
External Price |
|---|---|---|
| Whole Genome Shotgun or Metagenome Shotgun (PCR-free opt) | $140 | $230 |
| Mini Echo WGS DNA prep (minimum 8 samples for this price) | $8.80 | $13 |
| Mini Echo WGS DNA prep (minimum 80 samples for this price) | $3.60 | $5.30 |
| Mini Echo WGS DNA prep (minimum 350 samples for this price) | $2.30 | $3.40 |
| Stranded RNA-seq (with or without mRNA capture) | $155 | $255 |
| Small RNA-Seq | $120 | $185 |
| l0X Genomics Single Cell 3' Gene Expression (GEX) | $2,099 | $3,128 |
| 10X Genomics Single Cell ATAC-seq | $2,038 | $3,037 |
| 10X Genomics Single Cell Multiome (GEX + ATAC) | $3,692 | $5,502 |
| Phase Genomics Hi-C | $700 | $1,050 |
| Multiplexing of completed Illumina libraries | $12.50 | $12.50 |
| Multiplexing of completed Illumina libraries plus bead cleanup | $25 | $25 |
| BluePippin size selection | $75 | $112 |
| PacBio DNA Sequencing Library | $450 | $675 |
| Barcoded cDNA Synthesis for PacBio Iso-seq | $200 | $300 |
| 16S amplification for PacBio (price includes up to 95 samples) | $3,000 | $4,500 |
| 16S amplification for Illumina (price includes up to 95 samples) | $900 | $1,440 |
| Template Prep Services | Internal Price | External Price |
|---|---|---|
| Standard DNA extraction | $20 | $30 |
| Standard RNA extraction | $25 | $33 |
| DNase treatment of user-submitted RNA | $20 | $30 |
| High Molecular Weight (HMW) DNA Extraction (for long-read sequencing) | $150 | $225 |
| Bead cleanup of HMW DNA (for long-read sequencing) | $25 | $25 |
| High-throughput Kingfisher DNA Extraction (Plant, minimum 8 samples) | $3.25 | $4.22 |
| High-throughput Kingfisher DNA Extraction (Animal, minimum 8 samples) | $7.71 | $10.20 |
| 96-well Format Cleanup or Size Selection (minimum 8 samples) | $1.63 | $2.42 |
| 96-well Format Normalization | $1.50 | $2.23 |
| Speedvac concentration (price includes up to 4 plates) | $82 | $82 |
Omega Bio-Tek's TotalPure NGS Mag-Bind Beads can be used to purify both DNA and RNA. UO researchers can purchase aliquots of these beads from the GC3F for $7 per mL.
We have run a full series of tests to characterize their DNA size selection properties.
Click here
to download the full report!
We do our best to give an accurate estimate of turnaround time when service requests are submitted. These estimates are not guarantees and occasionally change due to unforeseen circumstances (instrument maintenance/repairs, staff sick leave, etc.). Please let us know if you have a hard deadline on project completion when you submit your service request and we will take measures to ensure that we either meet that deadline or inform you ASAP if circumstances beyond our control will cause us to miss it. Thanks!