Sample Prep Services

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Summary of Sample Prep Services

GC3F offers full-service sample preps for many popular sequencing library types, including custom projects by request! See the list of library and template prep services below. If the right prep for your project is not listed, reach out to us at genomics@uoregon.edu to discuss a custom project.

Internal pricing is available for University of Oregon, Oregon State University, Oregon Health and Science University, and Portland State University. External pricing applies for all other requesters. 

To submit a request for Sample Prep service, please register for an iLab account and then click "Request Services" from the top menu bar in iLab. It is important that your request has been submitted in iLab before you provide the physical samples to us.

CLICK HERE to submit a Sample Prep request!

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Shipping Instructions for Sample Prep Projects

Please deliver samples 288 Klamath Hall if you are an on-campus user. For off campus users, please mail samples frozen on dry ice in a well-protected vessel to the following address:

University of Oregon/GC3F
1318 Franklin Blvd
Room 273 Onyx Bridge
Eugene, OR 97403

Please ensure that your package will arrive at UO Mon-Fri. UO cannot accept deliveries on weekends or federal holidays.

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Library Prep Services

Internal Price

External Price
Whole Genome Shotgun or Metagenome Shotgun (PCR-free opt) $140 $230
Mini Echo WGS DNA prep (minimum 8 samples for this price) $8.80 $13
Mini Echo WGS DNA prep (minimum 80 samples for this price) $3.60 $5.30
Mini Echo WGS DNA prep (minimum 350 samples for this price) $2.30 $3.40
Stranded RNA-seq (with or without mRNA capture) $155 $255
Small RNA-Seq $120 $185
l0X Genomics Single Cell 3' Gene Expression (GEX) $1,686 $2,360
Phase Genomics Hi-C $700 $1,050
Multiplexing of completed Illumina libraries $12.50 $12.50
Multiplexing of completed Illumina libraries plus bead cleanup $25 $25
BluePippin size selection $75 $112
PacBio DNA Sequencing Library $450 $675
Barcoded cDNA Synthesis for PacBio Iso-seq $200 $300
16S amplification for PacBio (price includes up to 95 samples) $3,000 $4,500
16S amplification for Illumina (price includes up to 95 samples) $900 $1,440

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Template Prep Services Internal Price External Price
Standard DNA extraction $20 $30
Standard RNA extraction $25 $33
DNase treatment of user-submitted RNA $20 $30
High Molecular Weight (HMW) DNA Extraction (for long-read sequencing) $150 $225
Bead cleanup of HMW DNA (for long-read sequencing) $25 $25
High-throughput Kingfisher DNA Extraction (Plant, minimum 8 samples) $3.25 $4.22
High-throughput Kingfisher DNA Extraction (Animal, minimum 8 samples) $7.71 $10.20
96-well Format Cleanup or Size Selection (minimum 8 samples) $2.42 $2.42
96-well Format Normalization $1.50 $2.23
Speedvac concentration (price includes up to 4 plates) $82 $82

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Omega Bio-Tek's TotalPure NGS Mag-Bind Beads can be used to purify both DNA and RNA.  UO researchers can purchase aliquots of these beads from the GC3F for $7 per mL.

 

We have run a full series of tests to characterize their DNA size selection properties.

Click here to download the full report!

 

We do our best to give an accurate estimate of turnaround time when service requests are submitted. These estimates are not guarantees and occasionally change due to unforeseen circumstances (instrument maintenance/repairs, staff sick leave, etc.). Please let us know if you have a hard deadline on project completion when you submit your service request and we will take measures to ensure that we either meet that deadline or inform you ASAP if circumstances beyond our control will cause us to miss it. Thanks!