GC3F has provided PacBio sequencing since 2017 and has been a Certified Service Provider since 2021. With the acquisition of PacBio's Revio sequencer, we are now able to offer even more affordable and high-throughput sequencing. We are happy to work on "unconventional" projects and difficult-to-extract species.
Revio SPRQ
The Revio replaces our Sequel II and represents a significant increase in throughput, capable of running 16 SMRTcells per week. Revio data automatically includes 5mC at CpG sites (methylation data) for native DNA.
Revio SMRTcell yield depends on the library type:
- Whole-genome sequencing: 60-120Gb
- Amplicons <2 kb: 4-5 million reads
- Amplicons 2-5kb: 6-8 million reads
- Full-length RNA Kinnex: 50-60 million reads
- 16S Kinnex: 70-80 million reads
- 10X scRNA Kinnex: 100-120 million reads
Revio Submission Requirements
PacBio library prep and sequencing success is highly dependent on the quality of input DNA for library prep. When preparing a WGS library for sequencing, please ensure that your input DNA adheres to the input requirements listed under PacBio WGS Submission Requirements on our prep request page.